Table 3

Antibodies used for native ChIP (N-ChIP)

Antibody

Host

Product

Lot

Saturating quantity used for N-ChIPa


H3K9ac

Rabbit

Upstate, 07-352

DAM16924924

8 µl

H3K4me3

Rabbit

Upstate, 04-745

NG1680351

4 µl

H3K9me3

Rabbit

Abcam, Ab8898

733951

4 µl

H3K27me3

Rabbit

Diagenode, pAb-069-050

A29900242

8 µl


aSaturating quantities for H3K9ac and H3K9me3 antibodies were previously determined [56]. Saturating quantities for H3K4me3 and H3K27me3 were determined in this study by a titration experiment (data not shown).

Lepesant et al. Genome Biology 2012 13:R14   doi:10.1186/gb-2012-13-2-r14

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