Smed-H2B RNAi does not affect differentiated cell types and tissues. (a-f) WMISH of the neoblast markers Smedwi-1 and Smedwi-2 (also expressed in the CNS) (a), the nervous system markers h.10.2f and Smed-cintillo (arrows) (b), the digestive system markers Smed-laminin and Smed-porcn-1 (c), the protonephridial markers Smed-CAVII-1 and Smed-innexin-10 (d), the secretory cell markers Smed-mag-1 and Smed-tcen49 (e) and the body positional markers Smed-slit and Smed-ifb (f) in control(RNAi) (top panels) and Smed-H2B(RNAi) (bottom panels) animals 5 days after RNAi. Smed-H2B(RNAi) animals show a dramatic decrease of Smedwi-1 and Smedwi-2 signals 5 days after RNAi (a), but no significant difference for any differentiated cell marker (b-f). Anterior is to the left. Scale bars: 500 μm.
Solana et al. Genome Biology 2012 13:R19 doi:10.1186/gb-2012-13-3-r19