Validation of NB-expressed transcripts. (a) Dynamics of validated transcripts expression in irradiated samples (left) and Smed-H2B(RNAi) samples (right) relative to non-irradiated (left) and control(RNAi) (right) samples. (b-i) WMISH of the transcripts AAA.454ESTABI.20654 (Smed-tubulin-alpha-1) (b), AAA.454ESTABI.22202 (Smed-RAD51) (c), AAA.454ESTABI.21251 (Smed-mex-3) (d), AAA.454ESTABI.20351 (Smed-SWI/SNF-related) (e), AAA.454ESTABI.13593 (Smed-brd3) (f), AAA.454ESTABI.20699 (Smed-POP1) (g), AAA.454ESTABI.17101 (Smed-TDP2) (h) and AAA.454ESTABI.22368 (Smed-HD1) (i) in non-irradiated animals (top panels) and animals 2 days after irradiation (bottom panels). All validated transcripts, including the category 1 marker Smed-HD1, show a NB expression pattern that disappears in irradiated animals.
Solana et al. Genome Biology 2012 13:R19 doi:10.1186/gb-2012-13-3-r19