Figure 5.

Biogenesis of nat-siRNAs that depend on DCL3. (a-c) Accumulation of nat-siRNA1g13340 (a), nat-siRNA1g28100 (b) and nat-siRNA1g17745 (c)Total RNA (75 to 100 μg) of distinct dcl, rdr, polIV and polV mutants and corresponding WT are probed by LNA probes indicated in Figures 1 and 3. U6 was used as a loading control. Similar results were obtained in two biological repeats.