Figure 1.

Performance evaluation of five rRNA depletion methods. (a) Shown is the distribution of RNA-seq reads aligning to protein-coding sequences (CDS; blue), rRNA (red), and other regions (tRNA, non-coding RNA, small RNA, and intergenic regions; gray) for undepleted total RNA (top) and five rRNA depletion protocols. (b) The lengths of the black bars represent the coefficient of determination (R²) for RPKM values before and after rRNA depletion using different rRNA-depletion methods. Ribo-Zero, normalization using duplex-specific nuclease (DSN) and Ovation were tested on a 1:1:1 pool (by mass) of total RNA prepared from P. marinus, E. coli, and R. sphaeroides. MICROBExpress and mRNA-ONLY were performed on individual RNA preparations without pooling.