Figure 3.

Strand specificity of RNA-seq reads. Shown is a 17-kb window of the E. coli genome viewed with the Artemis browser [28]. The mapped reads aligning to the top strand (green) or bottom stand (purple) consistent with the direction of the annotated genes as represented by the blue boxes with arrows and corresponding gene ID numbers and operons below (for example, genes b3196 through b3206).