Figure 5.

Ribo-Zero depleted gene-expression profiles from intact and partially degraded human stool samples: technical reproducibility and correlation with total RNA-seq data. (a-d) Shown are double-log scatter plots and the coefficient of determination (R²) comparing RPKM values for 64,752 annotated CDSs (points in black) in 19 bacterial genomes between technical Ribo-Zero replicates (a,b) as well as with and without Ribo-Zero depletion (c,d). RNA extracted from stool A was largely intact (RIN = 9). RNA from stool B was partially degraded (RIN = 7). Input amount was 5 µg total RNA. Points on the axes represent CDSs without coverage in one of the two samples. The number of data points on the diagonal and on the axes is indicated. Points in green represent the unannotated rRNAs.