Additional file 1.

Figures S1 through S8 and legends. Figure S1: linear correlation of gene expression profiles before and after different rRNA depletion methods. Figure S2: detrimental effect of high GC content on enrichment by low-c0t normalization using duplex specific nuclease (DSN). Figure S3: fragmentation profile of total RNA prior to Ribo-Zero treatment. Figure S4: PER CDS detection sensitivity before and after Ribo-Zero treatment of intact and fragmented RNA. Figure S5: antisense read densities for two technical replicates of strand-specific RNA seq of Ribo-zero treated PER RNA. Figure S6: representation of bacterial species in sequencing data sets from two human stool samples. Figure S7: RNA-seq data for Prevotella copri, Bacteroides vulgatus, and Eubacterium rectale in stool A. Figure S8: RNA-seq data for Prevotella copri and Bacteroides vulgatus in stool B.

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Giannoukos et al. Genome Biology 2012 13:r23   doi:10.1186/gb-2012-13-3-r23