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Highly Accessed Review

Current challenges in de novo plant genome sequencing and assembly

Michael C Schatz*, Jan Witkowski and W Richard McCombie

Author Affiliations

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

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Genome Biology 2012, 13:243 doi:10.1186/gb-2012-13-4-243

Published: 27 April 2012

Abstract

Genome sequencing is now affordable, but assembling plant genomes de novo remains challenging. We assess the state of the art of assembly and review the best practices for the community.

Keywords:
DNA sequencing; genome assembly; plant genomics