Figure 1.

Identification of replication origins by deep sequencing of hydroxyurea-arrested cells. (a) The replication profile of a region of S. pombe dataset Sp1 containing three validated origins and several AT islands. The red curve shows the normalized replication fraction in HU-arrested cells. The blue dots show identified replication peaks. On the x-axis, black represents genes, orange represents AT-rich intergenes [17] and green represents validated origins (collated in [15]). (b) The difference between the G2 datasets form Sp1 and Sp2, demonstrating the magnitude of the noise in the datasets. (c) The replication profile of a region of S. octosporus dataset So1, as in (a). (d) The replication profile of a region of S. japonicus dataset Sj2 containing two cloned ARSs [28], as in (a). ORF, open reading frame.