Table 1

General features of the Bacillus cereus transcriptome, for strains ATCC 10987 and ATCC 14579

B. cereus ATCC 10987

B. cereus ATCC 14579


Annotated genes

6,014

5,497

Expressed genes

3,685

2,061

Operons

2,271

1,696

Genes with mRNA half-lives determined

2,745

1,675

TSS determined

1,222

1,002

Median 5' UTR length (nucleotides)

74.5

59

TES determined

918

717

Median 3' UTR length

31

27

Transcribed units with no annotation

20

17

Median gene half-life (minutes)

2.4

2.6

Minimum gene half-life (minutes)

0.6

0.6

Maximum gene half-life (minutes)

15 or above

15 or above

Median gene expression level (RPKM)

44.1

109

Minimum gene expression level (RPKM)

5.2

20.3

Maximum gene expression level (RPKM)

4 × 104

4 × 104


The criterion used for 'expressed genes' was having an expression level higher than 50 RPK in the t0 sample. The criterion used for 'operons' was a continuous stretch of overlapping reads covering one to several genes with the same orientation in the genome and for which the average sequence coverage was higher than five reads per base pair. RPKM, reads per kilobase per megabase.

Kristoffersen et al. Genome Biology 2012 13:R30   doi:10.1186/gb-2012-13-4-r30

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