Figure 6.

Chimeric read fraction estimated from double indexed libraries. P5 is the index inserted at the 5' end and P7 is the index inserted at the 3' end of the template DNA. Diamonds represent the fraction of the unmatched P5-P7 reads among all reads; the vertical line represents the fraction of the most abundant unmatched P5 component among all unmatched P5 components, while the rightmost column shows the average value of all P7 indexes. (a, b) The multiplexed sample sizes are 40 (a) and 90 (b), and each includes two independent libraries.