Fasting modulates the temporal regulation of circadian exons, and their relationship with transcript-level expression. (a) Schematic depicting the timing of important events during the two days of the fasting experiment; one day of LD followed by one day of DD. Colored boxes each depict 12 hour periods in the lighting schedule. White, day/lights on; black, night/dim red light; dark grey, subjective day/dim red light. ZT, Zeitgeber time (the time under an LD cycle); CT, circadian time (the corresponding time under a DD cycle). (b) Alternative splicing (AS) of four selected circadian exons. (c) Transcript-level expression for the same exons. For each combination of time point and feeding status, n = 3 and error bars represent standard error of the mean. The symbols above each pair of bars indicate the results of Bonferroni's post hoc test, comparing the two circadian time points in each feeding condition after a two-way ANOVA: Ns, not significant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.
McGlincy et al. Genome Biology 2012 13:R54 doi:10.1186/gb-2012-13-6-r54