Experimental validation of G0 TFs. Figure 5A: Viability of ΔTF strains in contrast to wildtype and negative controls, shown as a hierarchically clustered heat-map. Strains are shown vertically and time-points in days horizontally, coloured cells denote log fold change of viability (red - increased viability, blue - decreased viability). Asterisks denote statistical significance of fold change (linear regression, LR test, FDR p ≤ 0.05). Inviable strains with viability less than CFU = 0.005 are labeled with the symbol #. Time-point on day one corresponds to exponentially growing cells, measured seven hours after inoculation. Figure 5B: High-resolution viability curves of the first 72 hours of the time-course covering exponential growth, diauxic shift and post-diauxic phase. A subset of ΔTF strains (coloured solid lines), as well as wildtype strains (black solid lines) and controls (dashed and dotted lines) are shown. Figure 5C: G0 viability curve of super-wildtype strain Δbas1. Figure 5D: Viability curve of G0-essential strain Δ tup1. Figure 5E: Viability curve of positive control strain Δmip1. Figure 5F: Viability curve of negative control strain Δtup1. TF, transcription factor knockout strain; FDR, false discovery rate.
Reimand et al. Genome Biology 2012 13:R55 doi:10.1186/gb-2012-13-6-r55