The eukaryotic ribosomal DNA locus and the targets of amplicons studied here. (a) Part of the rRNA gene locus showing primer binding sites (not to scale). (b) Comparison of sequence complementarity for the 18S-0067a-deg primer against various eukaryotic groups. Clades belonging to Excavate (E) or Opisthokont (O) groups [27,41] are marked on the left. Apusozoa consist of ciliated protozoans . Cryptophyta and Haptophyceae are subgroups of algae . (c) Heat map indicating the edit distance (numbers of mismatches) between the 18S-0067a-deg primer and target sites in the indicated organisms. Colors are scaled to the relative proportion of each taxa represented at each edit distance.
Dollive et al. Genome Biology 2012 13:R60 doi:10.1186/gb-2012-13-7-r60