Figure 5.

Comparison of major eukaryotic microbes detected in human stool. Samples were assayed with the 18S rRNA gene amplicon, the ITS1 rRNA gene amplicon, and the shotgun genomic data in human stool. Human subjects and DNA purification methods are as indicated on the x-axis. Taxa are shown at the family level or as indicated. (a) 18S rRNA gene amplicon used to analyze stool samples. (b) 18S rRNA gene amplicon contamination controls. (c) ITS amplicon stool samples. (d) ITS rRNA gene amplicon contamination controls. The contamination controls in (b, d) consisted of DNA-free water passed through the full DNA purification, sequencing and analytical pipeline; six of eight samples yielded pyrosequence data, though with low read numbers.