Additional file 2.Comparison of PCR amplification reactions for DNA purified from stool using different methods. Average DNA yields were: PSP, 59.6 ng/μl; PowerSoil, 30.4 ng/μl; FastDNA extraction, 15.8 ng/μl; and the archaeal method, 12.7 ng/μl. PCR products were separated on an 0.8% agarose gel and stained with ethidium bromide. Top: amplification products generated using the 18S primer pair. Bottom: amplification products generated using the ITS1F-ITS2 primer pair. Format: PDF Size: 521KB Download file This file can be viewed with: Adobe Acrobat Reader Dollive et al. Genome Biology 2012 13:R60 doi:10.1186/gb-2012-13-7-r60 |