Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Open Access Highly Accessed Research

Staphylococcus epidermidis pan-genome sequence analysis reveals diversity of skin commensal and hospital infection-associated isolates

Sean Conlan1, Lilia A Mijares123, NISC Comparative Sequencing Program4, Jesse Becker4, Robert W Blakesley4, Gerard G Bouffard4, Shelise Brooks4, Holly Coleman4, Jyoti Gupta4, Natalie Gurson4, Morgan Park4, Brian Schmidt4, Pamela J Thomas4, Michael Otto5, Heidi H Kong6, Patrick R Murray2 and Julia A Segre1*

Author affiliations

1 National Human Genome Research Institute, NIH, Bethesda, MD 20892, USA

2 Department of Laboratory Medicine, NIH Clinical Center, NIH, Bethesda, MD 20892, USA

3 Department of Medical Research and Technology, University of Maryland School of Medicine, Baltimore, MD 21201, USA

4 NIH Intramural Sequencing Center, NIH, Rockville, MD 20852, USA

5 Laboratory of Human Bacterial Pathogenesis, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA

6 Dermatology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA

For all author emails, please log on.

Citation and License

Genome Biology 2012, 13:R64  doi:10.1186/gb-2012-13-7-r64

Published: 25 July 2012

Abstract

Background

While Staphylococcus epidermidis is commonly isolated from healthy human skin, it is also the most frequent cause of nosocomial infections on indwelling medical devices. Despite its importance, few genome sequences existed and the most frequent hospital-associated lineage, ST2, had not been fully sequenced.

Results

We cultivated 71 commensal S. epidermidis isolates from 15 skin sites and compared them with 28 nosocomial isolates from venous catheters and blood cultures. We produced 21 commensal and 9 nosocomial draft genomes, and annotated and compared their gene content, phylogenetic relatedness and biochemical functions. The commensal strains had an open pan-genome with 80% core genes and 20% variable genes. The variable genome was characterized by an overabundance of transposable elements, transcription factors and transporters. Biochemical diversity, as assayed by antibiotic resistance and in vitro biofilm formation, demonstrated the varied phenotypic consequences of this genomic diversity. The nosocomial isolates exhibited both large-scale rearrangements and single-nucleotide variation. We showed that S. epidermidis genomes separate into two phylogenetic groups, one consisting only of commensals. The formate dehydrogenase gene, present only in commensals, is a discriminatory marker between the two groups.

Conclusions

Commensal skin S. epidermidis have an open pan-genome and show considerable diversity between isolates, even when derived from a single individual or body site. For ST2, the most common nosocomial lineage, we detect variation between three independent isolates sequenced. Finally, phylogenetic analyses revealed a previously unrecognized group of S. epidermidis strains characterized by reduced virulence and formate dehydrogenase, which we propose as a clinical molecular marker.