Figure 2.

Characterization of promoter DNA methylation patterns during development through the MBT. (a) Average methylation profiles of HCPs and LCPs at pre-MBT, MBT and post-MBT stages. (b) Proportions of methylated HCPs and LCPs at pre-MBT, MBT and post-MBT stages; methylation is defined by detection of a methylation peak in the -1/0 kb window upstream of the TSS irrespective of downstream methylation (upstream (Up) and upstream/downstream (Up/Do) methylation; y-axis). ^a,bP = 0.0005; ^a,cP = 0.0027 (Fisher's test). (c) Percent increase in the numbers of genes methylated upstream of the TSS (Up-only; -1 to 0 kb), downstream of the TSS (Down-only; 0 to +1 kb), and both upstream and downstream (TSS), from pre-MBT to post-MBT. **P < 0.001 (Fisher) relative to increase in methylation in the whole regions analyzed (-5 to +1 kb).