Flowchart of Coev2Net. Left: Markov chain Monte Carlo (MCMC) sampling to generate synthetic homologous sequences for each complex template. Right: 1) for given query protein pairs, the best template (from the structural library) is identified by protein threading; 2) structural and sequence features are extracted from the interfacial alignment and residue correlations scored with respect to the profile PGM; and 3) a classifier gives the probability of interaction for the query protein pair.
Hosur et al. Genome Biology 2012 13:R76 doi:10.1186/gb-2012-13-8-r76