Table 1

Comparison of overlaps achieved by Braun et al. and our method when some of the initial Y2H interaction pairs are re-tested using LUMIER assay

Yeast strains implementation

Number validated (LUMIER)

Y2H PPIs

Percentage overlap

Y strain 2 m 1 reporter 1 mM_3-AT (Braun et al.)

19

33

57

Y strain 2 m 2 reporters 1 mM_3-AT (Braun et al.)

13

22

59

Y strain CEN 1 reporter 1 mM_3-AT (Braun et al.)

17

23

74

MaV CEN 2 reporters 20 mM_3-AT (Braun et al.)

9

14

64

Our prediction

14

19

74

Our prediction^a

10

11

91

^aThese pairs have LUMIER assay z-scores > 1.5.

Hosur et al. Genome Biology 2012 13:R76   doi:10.1186/gb-2012-13-8-r76