Additional file 1.

All mutants with deviating pHc at various pHex, and during respiratory growth. Mutants with aberrant pHc under the standard condition (glucose, pH 5.0) were subjected to growth in pH 3.0, 7.5, as well as 2% ethanol/2% glycerol pH 5.0. Mutants were pre-grown overnight under standard conditions except for the 2% ethanol/2% glycerol experiment, in which case mutants were pre-grown in 2% ethanol/2% glycerol because of the long adaptation time to non-fermentable carbon source conditions. Cells were re-inoculated in described conditions and grown for 4 hours prior to measurements. Mutants were measured at least six times at pH 5.0 and at least three times at all other conditions. Mutants with significantly low pHc in any condition are indicated in orange, while mutants with significantly high pHc are indicated in blue.

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Orij et al. Genome Biology 2012 13:R80   doi:10.1186/gb-2012-13-9-r80