Additional file 1.

Supplementary figures and tables. Figure S1: a diagram of each tissue-specific construct and expression of each transgenic strain as determined by GFP signal. Figure S2: demonstration that the GFP-tagged constructs rescue the mutant phenotypes of dpl-1 and lin-35 mutants. Figure S3: high correlation between replicates for each factor and between different experiments and transgenic EFL-1 binding mirrors endogenous binding in the L1 soma. Figure S4: Venn diagrams that show the comparison of called binding sites for each factor in each tissue between factors within a tissue and between tissues for a given factor. Figure S5: tissue-specific binding for a subset of germline-specific and soma-specific sites using ChIP-qPCR. Figure S6: an example of other direct target genes that still retain EFL-1 binding in lin-35 mutants. Additional binding profiles at the loci encoding various candidate small RNA pathway regulatory proteins not shown in Figure 5a. EFL-1 is not ectopically recruited to the promoters of germline-specific small RNA regulators. Figure S7: MEME analysis that shows that tissue-specific targets have distinct E2F binding motifs. Table S1: a list of all the strains used for ChIP-seq analyses. Table S2: number of reads for each sample and replicate used in the analyses. Also included is a section describing the materials and methods used for the additional data files.

Format: PDF Size: 982KB Download file

This file can be viewed with: Adobe Acrobat Reader

Kudron et al. Genome Biology 2013 14:R5   doi:10.1186/gb-2013-14-1-r5