Changes in FOXM1 binding induced by thiostrepton correlate with an altered gene expression profile. (a) Microarray analysis of gene expression changes following treatment of MCF7 cells with DMSO or thiostrepton (TS) for 6 h. Only genes with an FDR < 0.01 were considered, resulting in 5,638 differentially expressed genes. Heat map shows differentially expressed genes for the six replicates. (b) GeneGo analysis of the differentially expressed genes showing the top ten enriched biological processes. (c) Correlation between differentially bound FOXM1 peaks from DBA analysis and differentially expressed genes from the microarray analysis following thiostrepton treatment. Blue dots represent peaks within 10 kb of the gene TSS, grey dots < 50 kb from the TSS. (d) FOXM1 ChIP was performed on DMSO or thiostrepton-treated MCF7 cells and qPCR was used to check FOXM1 binding in regions identified in DBA analysis as significantly differentially bound. Data representative of triplicate experiments ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.
Sanders et al. Genome Biology 2013 14:R6 doi:10.1186/gb-2013-14-1-r6