Identification and quantification of the major miR-203 isoforms in skin by deep sequencing. (A) Table showing miR-203 with differential 5′ ends (shown in red). (B) Primer extension for miR-203 using RNA isolated from mouse total epidermis where upper band corresponds to miR-203 and lower corresponds to miR203iso. Dicer conditional knock out skin sample was used as a control. (C) Northern blot probed for miR-203. Left panel shows synthetic miRNA controls, and right panel shows mouse keratinocyte cultures where miR-203 was lowly expressed under the proliferative condition and highly induced in the differentiated condition. Staining of tRNAs by ethidium bromide (EtBr) is shown as the loading control.
Zhang et al. Genome Biology 2013 14:R109 doi:10.1186/gb-2013-14-10-r109