Factors affecting the strength of the transcription/Trep association. (A) Median G2-phase transcript levels (representing S-phase transcription; Additional file 1: Figure S4A) are shown for all cell cycle-regulated genes separated into 10 equally sized bins (deciles) by their Trep. For both yeast species, no correlation is observed for the first five bins, whereas a strong relationship is present for later Trep. (B) Consistent with the decile analysis, no oscillation is observed in the correlation between expression level and Trep for early Trep genes, while a strong oscillation is observed for late Trep genes. (C) Only weak oscillation is observed in the correlation between expression level and Trep for ORI-distal genes (>5 kb from the nearest ORI in budding yeast, or 10 kb in fission yeast), while a strong oscillation is observed for ORI-proximal genes.
Fraser Genome Biology 2013 14:R111 doi:10.1186/gb-2013-14-10-r111