Distribution of average %Xi expression levels shows a range of expression. (A) Ranked average %Xi expression (highest to left, lowest to right). Error bars represent the standard error of the mean while the color indicates the assigned genic XCI status. (B) For each gene, informative females are represented with a different shape based on the sample set (CEU, square; YRI, circle; FIB, triangle) and color based on XCI status (E, green; S, red). Note that the E and S ranges overlap because the E:S boundary differs in each female based on skewing. The genic XCI status was determined by calculating the percentage of females that escaped from XCI for each gene: subject (red), 0 to 22% of informative females escaped from XCI; variable escape (purple), 22 to 78% of informative females escaped from XCI: escape (green), 78 to 100% of informative females escaped from XCI. The number and percentage of all genes is given below each example as well as the number of novel calls in each category. The number of informative females for each example gene is: HDHD1A n = 39, CA5B n = 36, REPS2 n = 30, POF1B n = 22, CACNA1F n = 32 and RPGR n = 41.
Cotton et al. Genome Biology 2013 14:R122 doi:10.1186/gb-2013-14-11-r122