Open Access Highly Accessed Method

AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation

Sarah Aldridge1, Stephen Watt1, Michael A Quail2, Tim Rayner1, Margus Lukk1, Michael F Bimson3, Daniel Gaffney2 and Duncan T Odom12*

Author Affiliations

1 University of Cambridge, Cancer Research UK - Cambridge Institute, Li Ka Shing Centre, Robinson Way, Cambridge, CB2 0RE, UK

2 Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1HH, UK

3 Agilent Technologies UK Limited, Mead Road, Yarnton, Kidlington, Oxfordshire, OX5 1QU, United Kingdom

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Genome Biology 2013, 14:R124  doi:10.1186/gb-2013-14-11-r124

Published: 7 November 2013

Additional files

Additional file 1:

Supplementary Figures 1-8.

Format: ZIP Size: 1.7MB Download file

Open Data

Additional file 2:

Optimized and final settings for Agilent Bravo AHT-ChIP protocol.

Format: XLSX Size: 43KB Download file

Open Data

Additional file 3:

Agilent file for automated ChIP method.

Format: ZIP Size: 354KB Download file

Open Data

Additional file 4: Table S1:

MetaData, sequencing metrics, peak calls, file information for liver experiments.

Format: XLSX Size: 24KB Download file

Open Data

Additional file 5:

MetaData, sequencing metrics, peak calls, file information for cell-line experiments.

Format: XLSX Size: 72KB Download file

Open Data

Additional file 6:

Beckman. BMF file for Illumina library preparation method.

Format: BMF Size: 234KB Download file

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Additional file 7: Table S2:

Illumina library oligonucleotide sequences.

Format: XLSX Size: 49KB Download file

Open Data

Additional file 8: Table S3:

Primer sequences used in ChIP real-time PCR.

Format: XLSX Size: 9KB Download file

Open Data