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Open Access Research

Polysome profiling reveals translational control of gene expression in the human malaria parasite Plasmodium falciparum

Evelien M Bunnik1, Duk-Won Doug Chung1, Michael Hamilton1, Nadia Ponts12, Anita Saraf3, Jacques Prudhomme1, Laurence Florens3 and Karine G Le Roch14*

Author Affiliations

1 Department of Cell Biology and Neuroscience, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA

2 Current address: INRA Centre de Bordeaux Aquitaine, 33883 Villenave d’Ornon, Cedex, France

3 Stowers Institute for Medical Research, Kansas City, MO 64110, USA

4 Institute for Integrative Genome Biology, Center for Disease Vector Research, Department of Cell Biology and Neuroscience, University of California Riverside, 900 University Avenue, Riverside, CA 92521, USA

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Genome Biology 2013, 14:R128  doi:10.1186/gb-2013-14-11-r128

Published: 22 November 2013

Additional files

Additional file 1:

MudPIT analysis of polysome fractions.

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Open Data

Additional file 2: Figure S1:

Validation of sequencing data. Figure S2. Distribution of normalized read counts per gene for the ring, trophozoite and schizont stages of P. falciparum. Figure S3. Characterization of genes with high 5′ UTR coverage in polysome-associated mRNA. Figure S4. Stop codon readthrough candidate in P. falciparum. Table S2. Enriched gene ontology terms for steady-state mRNA expression clusters. Table S3. Enriched gene ontology terms for polysomal mRNA expression clusters. Table S6. Sequences of primers used for PCR and northern blot analyses.

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Additional file 3:

Novel introns and alternative splice variants detected in this study.

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Additional file 4:

Highly expressed introns.

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Additional file 5:

Raw and normalized exon counts for all genes, as well as numbers of reads mapped to the regions 500 bp upstream and 500 bp downstream of each gene. For genes containing introns, the number of reads mapped to each intron and the number of intron-spanning reads are also provided.

Format: XLSX Size: 4.4MB Download file

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