Histone modification states at repetitive elements. (a-d) ChIP of modified histone tails (H3K9me3, H3K4me3 and H3K27me3) followed by qPCR analysis in WT and Lsh−/− fibroblasts at indicated repeat sequences and beta-actin. (e-h) ChIP of modified histone tails followed by qPCR analysis in Dnmt3b+/− and Dnmt3b−/− MEFs at indicated repeat sequences and beta-actin. (i-l) ChIP of modified histone tails followed by qPCR analysis in p53−/− and p53−/− |Dnmt1−/− MEFs at indicated repeat sequences and beta-actin. Blue = WT fibroblasts; red = Lsh−/− fibroblasts; green = Dnmt3b+/− MEFs; light green = Dnmt3b−/− MEFs; orange = p53−/− MEFs; purple = p53−/− |Dnmt1−/− MEFs. Experiments represent triplicate analysis. Histone modification indicated above each plot. See Figure S6 in Additional file 1 for ChIP primer locations. Units are expressed as percentage enrichments that is, % (immunoprecipitated DNA/input DNA). Ig, immunoglobulin; IN, input DNA; IP, immunoprecipitated DNA.
Dunican et al. Genome Biology 2013 14:R146 doi:10.1186/gb-2013-14-12-r146