SOAPfuse: an algorithm for identifying fusion transcripts from paired-end RNA-Seq data
- Equal contributors
Genome Biology 2013, 14:R12 doi:10.1186/gb-2013-14-2-r12Published: 14 February 2013
We have developed a new method, SOAPfuse, to identify fusion transcripts from paired-end RNA-seq data. SOAPfuse applies an improved partial exhaustion algorithm to construct a library of fusion junction sequences, which can be used to efficiently identify fusion events, and employs a series of filters to nominate high-confidence fusion transcripts. Compared with other released tools, SOAPfuse achieves higher detection efficiency and consumed less computing resources. We applied SOAPfuse to RNA-seq data from two bladder cancer cell lines, and confirmed 15 fusion transcripts, including several novel events common to both cell lines. SOAPfuse is available at http://soap.genomics.org.cn/soapfuse.html.