Framework of SOAPfuse for discovering fusion events. (a) Model of the fusion event from Gene A and Gene B that is supported by span-reads and junc-reads. Gene A and reads mapped to it are in blue, and Gene B is in orange. The junction site is marked by a yellow dot. Two span-reads and two junc-reads are shown. (b) The four parts of the SOAPfuse algorithm: read alignment against the human genome reference and annotated transcript sequences; identifying candidate gene pairs by seeking span-reads; detection of predicted fusions; filtering fusions with several criteria to generate the final high-confidence fusion transcript list. Methods with central roles in the algorithm are indicated in red. Steps marked by an asterisk indicate key filtering steps.
Jia et al. Genome Biology 2013 14:R12 doi:10.1186/gb-2013-14-2-r12