Analysis of SNAP23-LRRC57 reported by deFuse. (a) SNAP23-LRRC57 analysis based on release 59 of the Ensembl annotation database. Sanger sequencing of RT-PCR amplicons of the SNAP23-LRRC57 fusion event reported by deFuse is shown. The upstream gene (LRRC57) is in blue, and the downstream part is in orange. Gene symbols are followed with their DNA strands. The downstream fusion part is the antisense strand sequence of exon 4 of LRRC57. (b) In the latest release (release 69) of the Ensembl annotation database, the downstream part of the SNAP23-LRRC57 fusion is annotated as part of exon 4 of SNAP23-017, one of the SNAP23 transcripts. The fusion SNAP23-LRRC57 reported by deFuse is in fact an alternative splicing event in gene SNAP23.
Jia et al. Genome Biology 2013 14:R12 doi:10.1186/gb-2013-14-2-r12