Nitrogen starvation induces specific RBP-crosslinking and mRNA changes associated with ribosomes and translation-related processes. (a) Global changes in 3' UTR crosslinking site coverage versus changes in the corresponding mRNA upon nitrogen starvation. Crosslinking sites on genes annotated with 'ribosome biogenesis' GO term are colored red. Dotted lines indicate ≥4-fold changes in crosslinking site coverage (vertical) or ≥2-fold change in mRNA expression (horizontal). (b) INO1 3' UTR contains one crosslinking site that increases in coverage upon nitrogen starvation and falls within a conserved block (blue diamond in (a)). INO1 mRNA expression is down-regulated upon nitrogen starvation. (c) AGP3 3' UTR contains three crosslinking sites that are lost and two crosslinking sites that appear upon nitrogen starvation (blue diamonds in (a)). AGP3 mRNA expression is up-regulated upon nitrogen starvation.
Freeberg et al. Genome Biology 2013 14:R13 doi:10.1186/gb-2013-14-2-r13