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Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery

Nikolaj Zuleger1, Shelagh Boyle2, David A Kelly1, Jose I de las Heras1, Vassiliki Lazou1, Nadia Korfali1, Dzmitry G Batrakou1, K Natalie Randles34, Glenn E Morris34, David J Harrison5, Wendy A Bickmore2 and Eric C Schirmer1*

Author Affiliations

1 The Wellcome Trust Centre for Cell Biology, University of Edinburgh, Mayfield Road, Edinburgh, EH9 3JR, UK

2 MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, Crewe Road, Edinburgh, EH4 2XU, UK

3 Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Twympath Lane, Oswestry, SY10 7AG, UK

4 Institute for Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST4 7QB, UK

5 School of Medicine, Medical andBiological Sciences, University of St Andrews, North Haugh, St Andrews, KY16 9TF, UK

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Genome Biology 2013, 14:R14  doi:10.1186/gb-2013-14-2-r14

Published: 15 February 2013

Additional files

Additional file 1:

Table S1 - statistics for Figures 1, 2, 5, 6, 7, and 9. Repositioning of lacO arrays (for Figures 1d and 2b, c) was assessed by comparing the number of arrays present within the outermost shell (shell 1) against the internal area (combined shells 3+4+5) between each NET and the mRFP control by means of the one-tailed chi-squared test. Chromosome repositioning was assessed by comparing the distribution of chromosome intensities within the periphery (combined shells 1 and 2) between each NET and the NLS-GFP (Figures 5b and 6c), scramble siRNA and NET siRNA (Figures 7b and 9e, f) or liver and kidney (Figure 9b) by means of the KS test. P-values for each sample are listed with a significance threshold of P < 0.01 for high stringency (red) or P < 0.05 for low stringency (blue).

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Additional file 2:

Table S2. Quantification of bands in western blots was performed using the LI-COR Odyssey Application Software version 3.0. Integrated intensities were calculated from an equal size box drawn around each band in each tissue (Table S2a). To assess the variation of protein abundance among tissues, the intensities were summed within each protein for all tissues, and then expressed as a percentage of the signal per tissue (Table S2b).

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