Table 2

Symbiotic phenotypes of Medicago plants inoculated with virB mutants of S. meliloti KH46c and S. medicae M2

Host plant

Inoculated strain

Nodule numbera

Nodule dry mass (mg)

Plant dry mass (mg)

Plant height (cm)

Chlorophyll content (SPAD unit)


M. truncatula

KH46c wild-type

79

6.6

208

12.2

44

A17

KH46c ΔvirB6-9

38*

4.3*

145*

9.5*

43

M2 wild-type

102

8.4

229

11.0

41

M2 ΔvirB6-9

51

6.2*

202

11.2

44

Uninoculated control

0

0

37

3.3

17

M. truncatula

KH46c wild-type

35

6.1

174

10.3

42

F83005-5

KH46c ΔvirB6-9

24

5.5

158

9.8

39

M2 wild-type

29

4.9

156

9.5

43

M2 ΔvirB6-9

22

6.7*

243*

10.7*

41

Uninoculated control

0

0

44

3.3

16

M. tricycla

KH46c wild-type

24

12.2

315

10.5

36

R108-C3

KH46c ΔvirB6-9

12*

9.9

230

10.3

34

M2 wild-type

11

2.8

33

4.2

19

M2 ΔvirB6-9

12

3.1

33

4.2

21

Uninoculated control

0

0

26

3.5

16

M. satvia cv

KH46c wild-type

56

1.6

95

8.5

54

Agate

KH46c ΔvirB6-9

42

6.8*

55

7.2

45*

M2 wild-type

31

2.5

69

13.7

31

M2 ΔvirB6-9

28

2.5

85

14.6

28*

Uninoculated control

0

0

79

12.5

21


aValues are per plant. The asterisk indicates a significant difference compared with the wild-type strain by t-test (P < 0.05) of three biological replicates.

Sugawara et al. Genome Biology 2013 14:R17   doi:10.1186/gb-2013-14-2-r17

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