ASH1, FSH co-localization correlates with gene expression. (A) Modified UCSC genome browser view of a representative genomic region containing silent (blue arrowheads) and active genes (red arrowheads). ASH1 and FSH tracks show data as described before; H3K4me3 track displays read coverage profile for H3K4me3 ChIP-seq experiment; mRNA track contains coverage profile obtained by short-read sequencing of mRNA (RNA-seq). FlyBase gene models are depicted below track area; arrowheads indicate direction of transcription; vertical dashed lines mark TSS positions. (B) Graphs show normalized ChIP-seq read density profiles across genes grouped according to their expression level (zero, low, medium, and high). (C) Comparison of ASH1, FSH ChIP-seq signals across intron-rich and intron-poor, active genes. Tracks display data as described before. Intron-rich, active genes show strong ASH1, FSH signals inside gene bodies in contrast to their intron-poor counterparts. Both gene classes share promoter proximal signals.
Kockmann et al. Genome Biology 2013 14:R18 doi:10.1186/gb-2013-14-2-r18