Additional file 5.

Information on functional analyses and comparison with existing caste expression data from other species. Tabs are as follows. 'Toth2010_Pmetricus_Pcan', mappings between P. metricus and P. canadensis transcripts and their respective expression levels and whether they are significantly differentially expressed in P. canadensis. 'Whitfield2003_50cDNAS', mappings between 50 most discriminative cDNAs for worker behaviors in A. mellifera from Whitfield et al. [18] and P. canadensis, with respective expression values. 'GO-Enrichment Differential Expressed (DE) Genes', list of over/under-represented GO terms for genes significantly up- and down-regulated in each caste comparison. Genes were categorized according to biological processes (P), molecular function (F) and cellular component (C). Most specific terms were retrieved with Blast2GO and correspond to the leaf nodes in the GO tree, this excludes cases where parent and child nodes may be included. Column labels are: Test (number of genes in the test set annotated with this GO term); Ref (number of genes in the ref set annotated with this GO term); notAnnotTest (number of genes in the test set NOT annotated with this GO term); notAnnotRef (number of genes in the reference set NOT annotated with this GO term), where 'test' set is the set of genes up-regulated in queens, and the 'ref' set those up-regulated genes in workers, and vice-versa. 'GO-Enrichment', list of over/under-represented GO terms for the P. canandensis genes that are conserved across the genomes of 6 eusocial insects. 'genes_with_Behavior_GOterm', genes classified with GO term thought to be relevant to behavior, their respective descriptions and direction of differential expression in P. canadensis. 'genes_accelerated_Pol_Bee_Ants', genes found accelerated in Polistes, bee and ants, and direction of differential expression in P. canadensis. 'genes_accelerated_polistes', genes found accelerated only in Polistes; and direction of differential expression in P. canadensis. 'phylogenetic marker identifiers', a list of the 93 marker genes used in the phylogenetic analyses. These correspond to phylomeDB codes in the Aphid phylome used as the reference markers. Sequences for these identifiers can be obtained from Huerta-Cepas et al. [57].

Format: XLS Size: 353KB Download file

This file can be viewed with: Microsoft Excel Viewer

Ferreira et al. Genome Biology 2013 14:R20   doi:10.1186/gb-2013-14-2-r20