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Redistribution of H3K27me3 upon DNA hypomethylation results in de-repression of Polycomb target genes

James P Reddington1, Sara M Perricone1, Colm E Nestor12, Judith Reichmann1, Neil A Youngson3, Masako Suzuki4, Diana Reinhardt1, Donncha S Dunican1, James G Prendergast1, Heidi Mjoseng1, Bernard H Ramsahoye5, Emma Whitelaw3, John M Greally4, Ian R Adams1, Wendy A Bickmore1 and Richard R Meehan12*

Author Affiliations

1 MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK

2 Breakthrough Breast Cancer Research Unit, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, UK

3 Queensland Institute of Medical Research, Herston, Queensland 4006, Australia

4 Departments of Genetics (Computational Genetics) and Center for Epigenomics, Albert Einstein College of Medicine, 1301 Morris Park Avenue, Bronx, NY, USA

5 Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK

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Genome Biology 2013, 14:R25  doi:10.1186/gb-2013-14-3-r25

Published: 25 March 2013

Additional files

Additional file 1:

A PDF document containing supplemental files for this paper. Included are eight supplemental figures (Figures S1 to S8), supplemental materials and methods (Text S1), and two supplemental tables (Tables S1 and S2).

Format: PDF Size: 2.7MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

An Excel spread sheet (XLS) showing normalized average enrichments and P-values observed at gene promoter regions by ChIP-chip for H3K27me3 and H3K4me3 in Dnmt1+/+ and Dnmt1-/- MEFs.

Format: XLS Size: 5.8MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

An Excel spread sheet (XLS) showing genomic windows (100 kb) that show differential H3K27me3 by ChIP-seq in Dnmt1+/+ and Dnmt1-/- MEFs, along with the number of H3K27me3 reads overlapping each window in each sample and their odds ratios.

Format: XLS Size: 184KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

An Excel spread sheet (XLSX - compressed in ZIP) showing genomic windows (1 kb) that show differential H3K27me3 by ChIP-seq in Dnmt1+/+ and Dnmt1-/- MEFs, along with the number of H3K27me3 reads overlapping each window in each sample and their odds ratios.

Format: ZIP Size: 19.1MB Download file

Open Data