Figure 2.

H3K27me3 redistribution in hypomethylated cells is associated with DNA methylation pattern. (A) Density plots showing the methylation of CpGs in Dnmt1^+/+ (upper) and Dnmt1^-/- (lower) MEFs. The methylation is plotted for all CpGs, CpGs within H3K27me3 down regions and H3K27me3 up regions defined by ChIP-seq. (B) Bisulfite sequencing of selected H3K27me3 down and H3K27me3 up regions in Dnmt1^+/+ and Dnmt1^-/- MEFs. Filled circles (black) represent 5mCpG and open circles represent CpG. Gaps indicate inconclusive base calling at a CpG site. The overall amount of 5mCpG as a percentage of total CpG in each amplicon is shown adjacent to each image. H3K27me3 down regions represent proximal promoter regions while H3K27me3 up regions represent intragenic or intergenic regions.