Figure 5.

H3K27me3 loss in hypomethylated cells is associated with ectopic gene expression. (A, B) Boxplots displaying the relative expression (log2) of H3K27me3 down genes (red) and all genes (white) between Dnmt1^+/+ and Dnmt1^-/- MEFs as measured by mRNA-seq (A) and expression microarray (B). **P <0.001 by Wilcoxon rank sum test. (C) Boxplot showing the difference in promoter enrichment for H3K4me3 between Dnmt1^+/+ and Dnmt1^-/- MEFs as measured by ChIP-chip. **P <0.001 by Wilcoxon rank sum test. (D) Venn diagram showing overlap between H3K27me3 down genes (red circle) and genes that show >2-fold increased expression in Dnmt1^-/- relative to Dnmt1^+/+ MEFs by mRNA-seq (yellow circle). The number of genes in each section of the diagram is shown. **P <0.001 as estimated by hyper-geometric testing for the enrichment of the overlap genes.