Open Access Research

RNA sequencing reveals sexually dimorphic gene expression before gonadal differentiation in chicken and allows comprehensive annotation of the W-chromosome

Katie L Ayers123, Nadia M Davidson1, Diana Demiyah4, Kelly N Roeszler1, Frank Grützner5, Andrew H Sinclair126, Alicia Oshlack1* and Craig A Smith126*

Author Affiliations

1 Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Road, Parkville 3054 Melbourne, VIC, Australia

2 Poultry Cooperative Research Centre, Armidale, NSW, Australia

3 Department of Genetics, The University of Melbourne, Parkville, 3054, Melbourne, VIC, Australia

4 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

5 The Robinson Institute, School of Molecular and Biomedical Science, University of Adelaide, 5005 Adelaide, SA, Australia

6 Department of Paediatrics, The University of Melbourne, Flemington Road, Parkville, 3054 Melbourne, VIC, Australia

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Genome Biology 2013, 14:R26  doi:10.1186/gb-2013-14-3-r26

Published: 25 March 2013

Additional files

Additional file 1:

Supplementary figures (1 to 10), supplementary methods, figure legends for supplementary figures and tables.

Format: DOCX Size: 9MB Download file

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Additional file 2:

Table S1a. Dimorphically expressed Ensembl annotated genes in the Blastoderm.

Format: XLSX Size: 102KB Download file

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Additional file 3:

Table S1b. Dimorphically expressed Ensembl annotated genes in the E4.5 gonads.

Format: XLSX Size: 127KB Download file

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Additional file 4:

Table S1c. Two tests for dimorphic expression.

Format: XLS Size: 130KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 5:

Table S2. Genes previously implicated in sexual reproduction or gonadogenesis in a variety of models.

Format: XLSX Size: 32KB Download file

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Additional file 6:

Table S3. Ensembl Identifiers for W-linked genes and their Z gametologues.

Format: XLSX Size: 42KB Download file

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Additional file 7:

W gene de-novo assembly sequences (fasta file).

Format: FA Size: 102KB Download file

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Additional file 8:

Z gene de-novo assembly sequences (fasta file).

Format: FA Size: 77KB Download file

Open Data