Comparison of the performances of Quartz-Seq, Quartz-Chip and other methods. (a) Box plot of Pearson correlation coefficients (PCCs) for the technical replication of Quartz-Seq and Smart-Seq with 10 pg diluted total RNA. We reanalyzed the following four original Smart-Seq datasets: mouse brain: MB; human brain (Nextera library preparation kit: HB (Nx); Universal human reference RNA: UHRR and UHRR with the Nextera library preparation kit: UHRR (Nx). The asterisk indicates the downsampling sequence reads from single-cell Quartz-Seq (paired-end (PE), 60 million reads, n = 3). (b) Box plot of PCCs between conventional RNA-seq and single-cell RNA-seq methods. (c) Comparison of our current and previous methods using 10 pg of total ES-cell RNA with GeneChip. Performance of (left) Quartz-Chip, and (right) the Kurimoto et al. method. The Kurimoto et al. data were reanalyzed using the original sets. The bar plots in the right panels show the numbers of genes that were detected with each method: the gray bars show the total number of genes, and the blue bars indicate the number of detected genes.
Sasagawa et al. Genome Biology 2013 14:R31 doi:10.1186/gb-2013-14-4-r31