Table 1 |
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Primers used in the various reactions |
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Primer |
Sequence 5'→' |
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RT primer (WTA) |
TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTTTTTTTTTTTTTTTTTTTTT |
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Tagging primer |
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTT |
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Suppression primer |
(NH2)-GTATAGAATTCGCGGCCGCTCGCGAT |
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TRSU |
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T |
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TRSI-2 |
(5'-phosphate)GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTT*G |
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TRSI-4 |
(5'-phosphate)GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTT*G |
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TRSI-5 |
(5'-phosphate)GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTT*G |
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TRSI-6 |
(5'-phosphate)GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTT*G |
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TRSI-7 |
(5'-phosphate)GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTT*G |
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TRSI-12 |
(5'-phosphate)GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTT*G |
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TPC1 |
AATGATACGGCGACCACCGA*G |
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TPC2 |
CAAGCAGAAGACGGCATACGA*G |
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RT primer (qPCR) |
TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTTTTTTTTTTTTTTTTTTTTT |
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qPCR, quantiative PCR, WTA, whole-transcript amplification. *Indicates phosphorothioate bonds |
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Sasagawa et al. Genome Biology 2013 14:R31 doi:10.1186/gb-2013-14-4-r31 |
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