Figure 4.

Correlating responses to PI-103 with phosphoproteomics data. The normalized intensities of phosphopeptides quantified across seven AML cell lines were correlated with their responses to the inhibition of proliferation after exposure to 1 μM PI-103, as measured by cell viability. (a) Phosphopeptides showing greater correlation with resistance or sensitivity to PI-103. (b) Relationship between normalized intensity of phosphorylated NBEAL1 and responses to PI-103. (c) Relationship between normalized intensity of phosphorylated DBNL and responses to PI-103. (d) Correlation of the sum of the intensities showing greater correlation with resistance. (e) Correlation of the sum of the intensities showing greater correlation with sensitivity. (f) Correlation of the ratio between phosphopeptide intensities showing greater correlation to resistance relative to those showing greater correlation with sensitivity. In each case, R refers to the Pearson correlation coefficient of the log2 transformed data; fold refers to the division of the intensities of the peptide group increased in the most resistant cell line by that of the most sensitive cell line; open squares are individual data points of three biological replicates; blue diamonds are the averages of the three replicates.