Additional file 14.
Primer extension assays on spliceosomal RNA U6. (A) Primer extension assay on spliceosomal RNA U6 detected documented 2'-O-methylation as well as potentially novel 2'-O-methylation sites. (B) Primer extension assay detected documented pseudouridine sites in U6. CTRL indicates the untreated sample, +CMC the sample treated with 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate (CMC).
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Kishore et al. Genome Biology 2013 14:R45 doi:10.1186/gb-2013-14-5-r45