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Open Access Research

5-Hydroxymethylcytosine is an essential intermediate of active DNA demethylation processes in primary human monocytes

Maja Klug13†, Sandra Schmidhofer1†, Claudia Gebhard1, Reinhard Andreesen2 and Michael Rehli1*

Author Affiliations

1 Department of Internal Medicine III, University Hospital Regensburg, D-93042 Regensburg, Germany

2 Regensburg Centre for Interventional Immunology (RCI), D-93042 Regensburg, Germany

3 German Cancer Research Center (DKFZ), D-69120 Heidelberg, Germany

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Genome Biology 2013, 14:R46 doi:10.1186/gb-2013-14-5-r46

Published: 26 May 2013

Additional files

Additional file 1:

Table S1. Word DocumentTitle of this dataset: Table S1, Oligonucleotide sequences used for PCRDescription of this dataset: Table S1 lists sequences of oligonucleotides for various PCR-based analyses.

Format: DOCX Size: 17KB Download file

Open Data

Additional File 2:

Table S2. Excel FileTitle of this dataset: Table S2, EpiTYPER resultsDescription of this dataset: Table S2 lists MassARRAY EpiTYPER results. EpiTYPER methylation ratios of individual CpG units in 12 amplicons covering six distinct genomic locations are given for all knockdown samples of different donors.

Format: XLSX Size: 51KB Download file

Open Data

Additional file 3:

Table S3. Excel FileTitle of this dataset: Table S2, EpiTYPER resultsDescription of this dataset: Table S2 lists MassARRAY EpiTYPER results. EpiTYPER methylation ratios of individual CpG units in 12 amplicons covering six distinct genomic locations are given for all time course samples of different donors.

Format: DOCX Size: 16KB Download file

Open Data

Additional file 4:

Table S4. Word DocumentTitle of this dataset: Table S2, Genomic position of analyzed CpG residuesDescription of this dataset: Table S2 lists genomic positions of CpG residues that were analyzed by MassARRAY or QUEST-qPCR.

Format: XLSX Size: 57KB Download file

Open Data

Additional file 5:

Supplementary Figures. PDFTitle of this dataset: Supplementary FiguresDescription of this dataset: Contains supplementary figures S1-5.

Format: PDF Size: 6.1MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data