Sum1-dependent recruitment of Sir2 to the PDC1 ORF. (a) ChIP assay showing Sir2-myc and Hst1-myc enrichment at the PDC1 ORF in WT and sum1Δ strains. (b) ChIP assay showing Sir2-myc enrichment in WT, sir3Δ, and sir4Δ strains. (c) ChIP assay showing Hst1-myc enrichment in WT and sir4Δ strains. The relative IP in each panel indicates the IP PCR signal divided by the input chromatin PCR signal. (d) Model of independent recruitment of Sir2 and Sum1 to the PDC1 ORF. In this model, Sir2 is recruited via Sum1, while Hst1 is recruited by an unidentified factor. Error bars represent standard deviation.
Li et al. Genome Biology 2013 14:R48 doi:10.1186/gb-2013-14-5-r48