Co-localization of Sir2, Hst1, and Sum1 with cohesin and condensin at Pol III-transcribed genes. (a) IGV screenshots showing enrichment of Sir2, Hst1, and Sum1 with three Pol III-transcribed genes. Hst2 behaves as a negative control. (b) ChIP assay confirming the enrichment of Sir2, Hst1 and Sum1. (c) The decreased binding of a cohesin subunit (Mcd1) and two condensin subunits (Smc4 and Brn1) to the tRNA genes when SIR2, HST1 or SUM1 are deleted. Cells were grown in YPD to the log phase. The relative IP in each panel indicates the IP PCR signal divided by the input chromatin PCR signal, for normalization. Error bars represent standard deviation.
Li et al. Genome Biology 2013 14:R48 doi:10.1186/gb-2013-14-5-r48