Enrichment of cohesin and condensin at the PDC1 gene and rDNA is dependent on SIR2, HST1, SUM1 and NPT1. (a) Zoomed out view of a 100 kb chromosome XII region with four ORFs with Sir2, Hst1, and Sum1 enrichment that coincide with a condensin peak (asterisks). (b) The reduced binding of cohesin and condensin subunits to the PDC1 ORF when SIR2, HST1 or SUM1 was deleted. Cells were grown in YPD to log phase. (c) Reduced association of cohesin (Mcd1) and condensin (Smc4) subunits to the rDNA when NPT1 was deleted. Scc2 is a subunit of the Scc2/4 cohesin loading complex. The relative IP in each panel indicates the IP PCR signal divided by the input chromatin PCR signal, for normalization. Error bars represent standard deviation.
Li et al. Genome Biology 2013 14:R48 doi:10.1186/gb-2013-14-5-r48